Tissue slices, suberized

Phenolic acids and aliphatic acids are both involved in the biosynthesis of suberin, and phenolic acids are not synthesized in tissue slices that do not undergo suberization. 

The time-course of deposition of aromatic monomers into the polymer laid down by suberizing tissue slices indicates that the phenolic matrix is deposited simultaneously with or slightly before the aliphatic components. The specific anionic peroxidase appeared with a time-course consistent with its involvement in the polymerization and deposition of the phenolic matrix of the suberin. Increase or decrease in suberin content involves similar changes in both the aliphatic and aromatic components and such changes are associated with the expected increase or decrease in the anionic peroxidase activity caused by physical or biological stress. 

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