Time-resolved surface enhanced fluorescence

Khoshravi et al. at Cybefluor used a different chelate, BCPDA, and measurement of fluorescence on the dried surface of microtitration wells [17]. They later enhanced the signal using alkaline phosphatase as a label and diflunisal phosphate as a substrate which, after cleavage by phosphatase, was detected by time-resolved fluorometry [18]. Commercial assays are not available, but a large number of sensitive research assays have been published by the group of Diamandis [19]. 

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