Time-resolved area normalized emission

Another powerful tool for examining this issue is the use of time-resolved fluorescence spectra, especially when combined with the technique of Time-Resolved Area Normalized Emission Spectra (TRANES) developed by Periasamy and coworkers [78-80]. In this method, separate decay curves are collected over a wide range of emission wavelengths and reconstructed into time-resolved spectra, which are then normalized to constant area. In this model-free approach, it is possible to deduce the nature of heterogeneity of the fluorescent species from the... [Pg.323]

Koti ASR, Periasamy N (2001) Application of time resolved area normalized emission spectroscopy to multicomponent systems. J Chem Phys 115(15) 7094-7099... [Pg.330]

Koti ASR, Krishna MMG, Periasamy N (2001) Time-resolved area-normalized emission spectroscopy (TRANES) a novel method for confirming emission from two excited states. J Phys Chem A 105(10) 1767-1771... [Pg.330]

A.S.R. Koti, M.M.G. Krishna, N. Periasamy, Time-Resolved Area-Normalized Emission Spectroscopy (TRANES) A Novel Method for Confirming Emission from Two Excited States, J. Phys. Chem A 105, 1767-1771 (2001)... [Pg.369]

Intensity/wavelength/time cross-sectional diagrams (or time-resolved fluorescence "contour" diagrams) are generated using a weighted nonlinear least squares polynomial surface procedure (20). Area-normalized TRE spectra can be used for convenient pictorial representation, since the absolute emission intensity of individual time-resolved spectra vary substantially with time after excitation. [Pg.372]

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