Time Needed to Reach Equilibrium, Applied Voltage

Time Needed to Reach Equilibrium, Applied Voltage 

The small LKB column 8101 should not be allowed to dissipate more than 2-3 W at the beginning, the large LKB column, 6-9 W. The maximum permissible effect always diminishes as electrofocusing proceeds. This is discussed in Section VI, 10. 

Since the conductivity throughout the column diminishes as the carrier ampholytes reach their isoelectric points, the applied voltage can be increased considerably. Between 600-1000 V can be applied, depending on the type of pH-gradient used. 

The time required for electrofocusing depends on the pH range and the voltage used. A narrow pH range takes more time than a broad one. This is because the proteins have to migrate longer distances while carry- 

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