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Thymidine hydroperoxide determination

The FOX assay applied to a skatole oxidation product isolated by HPLC gave a positive result, supporting the contention that it is skatolyl hydroperoxide (40) . Mixtures of 183 and the eight diastereoisomeric hydroperoxides 184 and 185 derived from thymidine (42), as shown in equation 64, can be separated and detected by RP-HPLC with UVD at 229 nm. Each isomer is determined by applying the FOX assay using a capillary reactor heated at 60 °C to provide sufficient time for total oxidation of the Fe(ll) ions, followed by UVD at 596 mn . A commercial kit based on the FOX assay for hydroperoxide determination in plasma, serum and tissue homogenizates appears in Table 2. [Pg.676]

The lifetimes of the 9 main thymidine hydroperoxides 5-7 that were determined in pure aqueous solutions at 37 °C were found to vary from 1 h for the two trans diastereomers of 6-hydroperoxides (Table 1) to several weeks for 5-(hydroperoxymethyl)-2 -deoxyuridine. It may be added that the cis diastereomers are more stable than the trans homologues, irrespective of the pair of diastereomeric hydroperoxides. Interestingly, at neutral or slightly acidic pH values a specific hydrolytic decomposition pathway was observed for the two classes of hydroxyhydroperoxides. Thus, it was found that the trans and... [Pg.930]

The lifetimes of the 9 main thymidine hydroperoxides 5-7 that were determined in pure aqueous solutions at 37 °C were found to vary from 1 h for the two trans diastereomers of... [Pg.930]


See other pages where Thymidine hydroperoxide determination is mentioned: [Pg.1493]    [Pg.54]    [Pg.343]    [Pg.16]    [Pg.678]    [Pg.922]    [Pg.678]    [Pg.922]   
See also in sourсe #XX -- [ Pg.676 , Pg.695 ]




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Thymidine

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