Thymidine-3 , 5 -diphosphate staphylococcal nuclease

Figure 12-29 Drawing showing the hydrogen-bonding interactions between the guanidinium ions of arginines 35 and 87 of the micrococcal (staphylococcal) nuclease with the 5 -phosphate of the inhibitor thymidine 3, 5 -diphosphate in the complex of E + I + Ca2+. A possible mechanism is illustrated. A hydroxyl ion bound to Ca2+ carries out an in-line attack on the phosphorus. See Libson et al.S26...

Fig. 12. Active site of wild-type staphylococcal nuclease with bound active site ligands Ca and thymidine 3, 5 -diphosphate. Reproduced with permission from Ref. 88.

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