Three step purification of a recombinant enzyme

This example demonstrates one of the most common purification strategies  

IEX for capture, HIC for intermediate purification and GF for the polishing step. The objective of this purification was to obtain highly purified protein for crystallisation and structural determination. 

A more detailed description of this work can be found in Application Note 

Deacetoxycephalosporin C synthase (DAOCS), an oxygen-sensitive enzyme. 

Recombinant protein over-expressed in soluble form in the cytoplasm E. coli bacteria 

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