Thin-layer chromatography chlorophyll analysis

High performance liquid chromatography (HPLC) has been by far the most important method for separating chlorophylls. Open column chromatography and thin layer chromatography are still used for clean-up procedures to isolate and separate carotenoids and other lipids from chlorophylls and for preparative applications, but both are losing importance for analytical purposes due to their low resolution and have been replaced by more effective techniques like solid phase, supercritical fluid extraction and counter current chromatography. The whole analysis should be as brief as possible, since each additional step is a potential source of epimers and allomers. 

Zonneveld, H., Klop, W., and Gorin, N., Analysis of unboiled and boiled beans for chlorophylls and pheophytins by thin-layer chromatography and fluorimetry comparison... 

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