Thermostable alanine racemase

Structure of Thermostable Alanine Racemase of Bacillus stearothermophilus... 

The subunit dissociation is usually accompanied by destruction of the secondary structures when thermolabile proteins are used. The thermostable alanine racemase is very useful for studying the mechanism of subunit dissociation and protein unfolding. 

A simple procedure was established for the synthesis of various D-amino adds by means of four types of thermostable enzymes alanine racemase, D-amino acid aminotransferase 49, 501, L-alanine dehydrogenase 51, and formate dehydrogenase (Fig. 17-4) 171. The commercial preparation of formate dehydrogenase from Candida boidinii used by Wichmanri et al. 38 is not sufficiently stable. However, Galkin et al.1521 doned and expressed the gene of thermostable formate dehydrogenase in E. coli. 

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