Thermal processing partial unfolding

During the forward folding pathway the newly synthesized chain forms a partially folded single chain intermediate (5435). This folds further to a species with sufficient structure for chain recognition. Three of these intermediates associate into a protrimer in which the chains are associated but not fully folded (36). This species then folds further to the thermostable detergent and protease resistant native trimer. There are no known covalent modifications in the process. A similar process occurs in refolding urea denatured chains in vitro (37). Thermal Unfolding Pathway of Tailspike... 

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