The Time Course of Changes in Receptor Occupancy

3 THE TIME COURSE OF CHANGES IN RECEPTOR OCCUPANCY 1.3.1 Introduction 

For these reasons, kinetic measurements are now usually done with isolated cells (e.g., a single neuron or a muscle fiber) or even a patch of cell membrane held on the tip of a suitable microelectrode. Another approach is to work with a cell membrane preparation and examine direcdy the rate at which a suitable radioligand combines with, or dissociates from, the receptors that the membrane carries. Our next task is to consider what binding kinetics might be expected under such conditions. 

---