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The Development of Matrices and Surfaces

Additionally, the nitrocellulose surface provides an opportunity to carry out enzymatic and chemical reactions directly on the sample foil. These are discussed below, and in more detail later in Chapter 10, and include carboxy and amino-peptidase reactions that can be used to produce peptide ladder mixtures for determining amino acid sequence or the location of post-translational modifications. Following the on-foil reaction, the enzyme can be washed off prior to PDMS analysis of the resulting products. [Pg.85]

FIGURE 4.9 Positive ion PDMS spectra of porcine insulin (MW = 5778). (a) 17 nmol of electrosprayed insulin, (b) 3.5 nmol of insulin electrosprayed with 200 nmol reduced glutathione, and (c) 0.8 nmol of insulin adsorbed to nitrocellulose from a 0.1 % trifluroacetic acid solution. (Reprinted with permission from reference 23). [Pg.86]

FIGURE 4.10 Peak widths of the singly, doubly, and triply charged molecular ions of (a) lactal-bumin and (b) lysozyme, as a function of pH. (Reprinted with permission from reference 26). [Pg.87]

TABLE 4.1 Peptides grouped by numbers of amino acids.  [Pg.88]

Cal/mole, calculated according to Bull, H.B. (1974) 665. Breese, K, Arch. Biochem. Biophys. 161  [Pg.88]


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