The Chromatogram and Its Meaning

The substances eluted are transported by the mobile phase to the detector and are registered as Gaussian curves (peaks). The peaks give qualitative and quantitative information on the mixture investigated. 

At a constant flow rate, tg is directly proportional to the retention volume Vg. 

The retention volume shows how much mobile phase has passed through the separating system until half of the substance has eluted (peak maximum ). 

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