The Choice of Separation Medium

A particle size range should be chosen that gives the desired degree of resolution for the required chromatographic productivity (sample load and flow-rate). Once again, a series of trial runs may be necessary to find the optimal gel. The relationship 

Medium Matrix Dry particle Approximate separation Supplier 

Sephadex G-25 Medium Dextran 50-150 1x10 -5x10 Pharmacia 

Sephadex G-25 Fine Dextran 20-80 1x10 -5x10 Pharmacia 

BioGel P-6 Fine Polyacrylamide 45-90 Ixl0 -6xl0 BioRad 

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