The Cell-Free Protein Expression Systems RTS

The second platform (RTS 500 HY) is designed for the production of proteins on a preparative scale. It is based on the CECF principle and utilizes a device with two chambers (Fig. 1.7). This design, with a proper choice of reaction conditions, gives a reaction time of 24 h, yielding up to 6 mg of protein per ml. A scaled-up version is also available (RTS 9000 HY), providing up to 50 mg protein per run. 

In all formats, the amino acids are supplied separately, so they can be conveniently exchanged for labeled ones. 

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