The catalytic properties of immobilized enzyme

Our group has demonstrated that the catalytic properties of immobilized enzymes can be manipulated by the temperature-dependent swelling behavior of the microgel. The hydrolytic activity of adsorbed and native P-D-glucosidase was determined as a function of temperature. Desorption of immobilized enzyme upon... 

Thus, the results shown here demonstrate that thermosensitive microgel particles can serve as superior carriers for the adsorption of enzymes in which the activity of adsorbed enzymes are preserved. The catalytic activity of adsorbed P-D-glucosidase from almonds is increased by a factor of more than three. Moreover, the catalytic properties of immobilized enzymes can be manipulated by the volume transition of the microgel. Hence, such microgels present a novel class of active nanoreactors for biocatalysis. 

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