The bicinchoninic assay

This is a modification of the Folin method, without the Folin reagent In this case the Cu formed does not mediate reduction of the Folin reagent but is chelated by bicinchoninic add. 

Solutions (A) and (B) are stable for several months at room temperature. To use, mix reagents (A) and (B) in a ratio of 1 50 to form the bidnchonintc reagent. 

The sample should contain 5-40 jig protein in 0.2 ml water or buffer. 

1 Construct a standard curve by adding 2.3 ml bicinchoninic reagent to samples of 0.2 ml of BSA (1-40 M-g) in water. 

4 Read the Asa. of each sample against a blank of water or buffer plus reagents. Note that 10 (j-g of protein gives an of approximately 0.1. 

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