The basics of quantitation

The area or integral of an NMR signal is directly proportional to the molar amount of the species giving rise to the signal. Thus, 

Where Aj and A2 are the areas of the two signals andlVj and N2 are the respective numbers of protons generating the signals. For cases where protons in the same molecule are being quantitated, Ni and N2 represent the number of protons in the functional groups, n and /I2. In general for two species we can say that 

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