Tetrasaccharide synthesis

Iteration and sequential glycosidation in a stepwise manner or by block synthesis (e.g., disaccharide donor and disaccharide acceptor for tetrasaccharide synthesis)... 

Glycosynthase activity for tetrasaccharide synthesis from a-lactosyl fluoride and p-nitrophenyl /3-cellobioside (PNPC) was measured for the Humicola insolens Cel7B variants in sodium phosphate buffer, pH 7.0, at room temperature. Specific activities were determined by measuring the fluoride ion release rate by a fluoride ion selective electrode. The protein purification yields are the yield of purified protein as determined by western analysis from total cell culture. 

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