Testis steroid hydroxylases

Soucy P, Lacoste L, Luu-The V (2003) Assessment of porcine and human 16-ene-synthase, a third activity of P450cl7, in the formation of an andro-stenol precursor. Eur J Biochem 270 1349-1355 Fluck CE, Miller WL, Auchus RJ (2003) The 17, 20-lyase activity of cytochrome P450cl7 from human fetal testis favors the D steroidogenic pathway. J Clin Endocrinol Metab 88 3762-3766 Yoshimoto FK, Zhou Y, Peng HM, Stidd D, Yo-shimoto JA, Sharma KK, Matthew S, Auchus RJ (2012) Minor activities and transition state properties of the human steroid hydroxylases cytochromes P450cl7 and P450c21, from reactions observed with deuterium-labeled substrates. Biochemistry 51 7064-7077... 

Fevold HR, Lorence MC, McCarthy JL, Trant JM, Kagimoto M, Waterman MR, Mason JI (1989) Rat P450-17a from testis characterization of a full-length cDNA encoding a unique steroid hydroxylase capable of catalyzing both A and A -steroid-17,20-lyase reactions. Mol Endocrinol 3 968-975... 

The same electron transfer system operates in the 22-, 20-, and 18-hydroxylations as well as in the 11 -hydroxylation. Further, the identical electron transfer system appears to be pertinent to the system of steroid hydroxylation in testis and ovary. 22-, 20-, 18-, and 11 p-hydroxy-lases are all distributed in the mitochondrial fraction of adrenal cortex, whereas 21- and 17-hydroxylases are located in the microsomal fraction. The electron transfer system in the microsomal steroid hydroxylation has not yet been established. 

Chung, B., J. Picado-Leonard, M. Haniu, M. Bienkowski, RE Hall, J.E. Shively etal. (1987). Cytochrome P450c 17 (steroid 17a-hydroxylase/17, 20 lyase) Cloning of human adrenal and testis cDNAs indicates the same gene is expressed in both tissues. Proc. Natl. Acad. Sci. USA 84,407-411. 

Nakajin S, Hall PF. Microsomal cytochrome P-450 from neonatal pig testis. Purification and properties of a C-21 steroid side-ohain cleavage system (17a-hydroxylase-C17,20-lyase). J Biol Chem 1981 256 3871-3876. 

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