Test methods, standardization algal

Algae are cultured and exposed to the test substance in a nutrient-enriched medium. Tests consistent with OECD Test Guideline 201 (Algal growth inhibition) should be used. Standard test methods employ a cell density in the inoculum in order to ensure exponential growth through the test, usually 3 to 4 days duration. 

ASTM G 29 [498] Standard Practice for Determining Algal Resistance of Plastic Films ASTM E 154 [499] Standard Test Method for Water Penetration and Leakage Through Masonry... 

Bioassay. Toxicity of the materials was measured by the standard mouse bioassay for paralytic shellfish toxins and expressed by mouse unit (MU) as defined by the method (16). For testing the low toxin levels of algal specimens, extracts were treated with a charcoal column prior to injection into mice. 

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