Test method for pulmonary sensitization

Using an indelible ink marker, mark seven injection sites along each shaved side of the animals the sites should be approximately 5 cm apart. 

Prepare the challenge antigen solutions by making 500 pl/ml solutions of PA-PGSA or GPSA in 1.0% Evans blue dye. 

Weigh each animal and lightly ether anesthetize 4 h after the i.d. injections. 

Using the 21-gauge needle, i.c. inject 2.0 ml/kg of the challenge antigen solutions intracutaneously into the appropriate animals (i.e., a 400-g guinea pig is i.c. injected with 0.8 ml of challenge solution.) 

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