Ternary solvent diagram, mobile phase

Figure 1. Ternary solvent diagram indicating mobile phase concentrations for isocratic data points. Reproduced from Ref. 22. Copyright 1985, American Chemical Society.

Fig. 1.34. The selectiviiy window diagram for ihe optimisaiion of isocratic ternary mobile phases meihanol-acetoniirile-water. The maximum separation factor ri j in reversed-phasc separation of twelve phenylurea herbicides is searched for. X = concentration of acetonitrile related to the concentration sum of the organic. solvents in ternary mobile pha.ses. Column and sample compounds as in Fig. 1.33.

The composition of mixed mobile phases for ternary or quaternary isoselective gradient elution can be optimized using overlapping resolution mapping strategy to adjust optimum separation selectivity based on seven or more initial experiments with solvent mixtures of approximately equal elution strengths. Based on the retention data from the initial experiments, either 3-D diagrams or contour resolution... 

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