Termination of Animals

Recent research has indicated that any study using laboratory animals must be conducted based on principles of good laboratory practice. This is particularly important while handling and during termination of laboratory animals. All animals should be treated humanely (Tables 12-6 and 12-7). For more details, refer to Chapter 18. 

For the safe evaluation of chemicals and drugs, several animal species are used (e.g., mice, rats, cats, dogs, nonhuman primates). Test chemical(s) are administered through different routes such as oral, dermal, intravenous, intraperitoneal, 

TABLE 12-6 Termination of Mouse and Albino Rat by Euthanasia (by gases) 

Species Carbon Monoxide Carbon Dioxide Carbon Dioxide and Chloroform Nitrogen Flushing Argon Flushing 

Source Modified from Laboratory Animal Information Service and Centre, National Institute of Nutrition, Indian Council of Medical Research, Government of India, Hyderabad, India. 

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