Teriary structure

Lindskog and Malmstrom (1962) first succeeded in removing the zinc under sufficiently mild conditions to avoid denaturation. The zinc was complexed with phenan-throline and the resulting complex removed by dialysis. The apoenzyme was catalytically inactive and the activity could be restored by addition of one mole of zinc per mole of apoenzyme. ORD studies have shown that the apoenzyme and the native enzyme have the same gross teriary structure, confirming that the function of the zinc is not to stabilise the tertiary structure, but it is directly involved in the catalytic activity of the enzyme. 

Table 12.5-2. Evolutionary classification of peptidases into families and clans based on primary and teriary structure.

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