Temperature modulated reversed phase

All separations were performed on 625 or 626 LC Systems with a 715 Ultra WISP or 717 Plus Autosampler and a Temperature Control Module (all from Waters, Milford, MA). HPLC columns used for separations were Delta-Pak C18 300A reversed-phase columns (2.0 x 150mm or 3.9 x 150mm). 

Fig. 1 Flow diagram of an HPLC system with postcolumn iodine-azide reaction detection. A, Ion and ion-pair chromatography, and B, reversed-phase chromatography 1, mobile phase 2, pump 3, injection valve 4, HPLC column 5, mixing tee 6, sodium azide solution 7, iodine solution 8, reaction tube 9, thermostat 10, LC spectrophotometer 11, recorder or computer as a recorder 12, acetonitrile 13, postcolumn reaction module 14, temperature control system 15, bus Sat/In module 16, water.

The simulation and optimization modules are used for the simulation of various experiments and for the optimization of isocratic and gradient HPLC methods. Parameters that can be optimized are concentration of organic solvent in the mobile phase, linear and multi-segment gradient profile, pH value, temperature, type of reversed-phase column, and organic solvent. 

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