TBLASTN search

Pig. 2. This figure shows the result of performing a TBLASTN search against the nonre-dundant (NR) GenBank database using the peptide sequence of the tomato ZDS minus the transit peptide sequence (17). A TBLASTN search compares the amino acid input to the translated nucleotide sequences contained in the database (55,56). Presented by the permission of NCBI. 

There are three varieties of translated BLAST search tblastn, blastx, and tblastx. In the first variant, tblastn, a protein sequence query is compared to the six-frame translations of the sequences in a nucleotide database. In the second variant, blastx, a nucleotide sequence query is translated in six reading frames, and the resulting six-protein sequences are compared, in turn, to those in a protein sequence database. In the third variant, tblastx, both the query and database subject nucleotide sequences are translated in six reading frames, after which 36 (6 x 6) protein blastp comparisons are made. Protein sequences... 

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