Tandom mass spectrometry

LC-ESI-MS/MS analysis. To examine the potential PTM of a protein after MADLI-TOF mass, a liquid chromatography-tandom mass spectrometry (LC-ESI-MS/MS) is run to obtain information-dependent acquisition (IDA) data. Alternatively, an LC-ESI-MS/MS analysis can be performed without prior MALDI-TOF. However, it is the most common practice to first conduct a MALDI-TOF mass to determine the amount of sample to be loaded on a subsequent LC-ESI-MS/MS. The IDA data could then be searched at Mascot MS/MS data search (available online at http //www.matrixscience.com/) by selecting several parameters, such as protein data bank (i.e., NCBI and MSDB), the species, the fixed modification (i.e., carbamidomethyl cystein), the variable modifications, and the mass tolerance. The search result will generate information about the protein. The reconstructed MS/MS spectrum along with the calculated mass value of product ions matched in the original MS/MS spectrum can be obtained, which will show the residue modified by PTM. To confirm the modification, manual analysis of the MS/MS data is essential. 

Dieckhaus CM, Fernandez-Metzler CL, King R, Krolikowski PH, Baillie TA. Negative ion tandom mass spectrometry for the detection of glutathione conjugates. Chem Res Toxicol 2005 18 630-638. 

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See also in sourсe #XX -- [ , , , , , ]

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