Tandem double-focusing mass analysers

Positive ion fast atom bombardment (FAB) mass spectra were obtained with the JEOL HXllO/HXllO tandem double-focusing mass spectrometer with JEOL gun and collisionally induced decomposition (CID) MS/MS. The JEOL instrument was operated at +10 kV with -20 kV postacceleration at the detector. The xenon neutral beam had 6 kV acceleration from the JEOL gun. CID MS/MS was performed with 1 1000 resolution in both MS-1 and MS-2. Helium was used as the collision gas at a pressure sufficient to reduce precursor ion abundance by 75%. Samples were dissolved in methanol-glycerol (1 1, v/v) for all FABMS analyses. 

Fig. 5 Statistical evaluation of LC-MS-based methods for tropane alkaloids referred in this chapter. (a) Relative frequency of ionization methods. +APCI positive atmospheric pressure chemical ionization, +ESI positive electrospray ionization, FAB fast atom bombardment, +TSP positive thermospray, (b) Relative frequency of scan modes used. MS full scan MS, MS/MS tandem mass spectrometry (product ion scan), MRM multiple reaction monitoring, SIM selected ion monitoring, (c) Relative frequency of mass analysers used. EBQtQ2 double focusing sector field mass spectrometer, IT ion trap, QqQ triple quadrupole, SQ single quadrupole. Considered publications were found by PubMed data-based search and references cited in these articles...

Tandem mass spectrometry is applicable to all types of biomolecules but typically requires specialized mass spectrometry instrumentation for implementation. The most common tandem mass spectrometer is a triple quadrupole instrument, wherein the first and third quadrupoles are used as mass analysers and the middle quadrupole is used as a collision chamber. MS/MS can be performed with double focusing sector instruments and in some cases with specialized TOF mass analysers. Quadrupole ion traps and FTICR mass spectrometers are ideally suited for MS/MS experiments, and due to the operational characteristics of these mass analysers... 

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