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Tandem bioluminescent enzyme immunoassay

Ito, K. Nakagawa, K. Murakami, S. Arakawa, H. Maeda, M. Highly sensitive simultaneous bioluminescent measurement of acetate kinase and pyruvate phosphate dikinase activities using a firefly luciferase-luciferin reaction and its application to a tandem bioluminescent enzyme immunoassay. Anal. Sci., 19, 105-109 (2003)... [Pg.274]

DEVELOPMENT OF TANDEM BIOLUMINESCENT ENZYME IMMUNOASSAY FOR ANGIOTENSIN I AND ENDOTHELIN-1... [Pg.471]

We developed a simultaneous bioluminescent assay of acetate kinase (AK) and pyruvate phosphate dikinase (PPDK). In the method, the detection limits (blank + 3SD) of AK and PPDK were 1.03x10 and 2.05x10 mol/assay, respectively. Previously, we successfully applied a tandem bioluminescent enzyme immunoassay (BL-EIA) for simultaneous measurement of insulin and c-peptide. In this study, we also applied the method to tandem BL-EIA for Angiotensin I and Endothelin-1, which are hypertension related peptides. The tandem BL-EIA used a competitive immuno-reaction for Angiotensin I and sandwich inununo-reaction for Endothelin-1. [Pg.471]

See other pages where Tandem bioluminescent enzyme immunoassay is mentioned: [Pg.461]    [Pg.461]    [Pg.459]   
See also in sourсe #XX -- [ Pg.471 ]



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