T-even phage DNA

Figure 9-27. Buoyant densities (25°C) of native DNA s in CS2SO4 (A) and CsCl (B) gradients as a function of base composition ( , solid lines), expressed as mole percent of G + C or G + HMC, or as a function of the glucose to HMC ratio (mole %), the latter for T-even phage DNA (O, dashed lines). Denatured E. coli DNA (Den. E COLI, 0) was prepared by heating DNA (10 jag/ml) for 10 minutes at 96 to 100°C in 0.02M sodium citrate (pH = 7.8) and rapidiy chilling to 0°C. Based largely on the data of R. L. Erikson and W. Szybalski, Virology, 22 111 (1964). [From W. Szybalski, in Methods of Enzymology, Vol. 12B, Academic Press, New York, 1968, p. 330.]...

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