T-butyl 6-chloro-3,5-dioxohexanoate

Regioselective Reduction of t-Butyl 6-chloro-3,5-dioxohexanoate to the Corresponding Enantiopure (S)-S-Hydroxy Compound... 

After characterization of the systems, biotra ns formations were performed to produce chiral alcohols using 10 mM acetophenone, 15 mM 2,5-hexanedione, and 25 mM t-butyl 6-chloro-3,5-dioxohexanoate as substrates (Scheme 2.2.4.5). 

The specific cell activities for the reduction of 10 mM acetophenone, 15 mM 2,5-hexanedione, and 25 mM t-butyl 6-chloro-3,5-dioxohexanoate by E. coli BL21(DE3)/ pAW-3 and E. coli BL21(DE3)/pAW-4 are listed in Table 2.2.4.3, which shows that the reduction of acetophenone is the most efficient one, and that excellent specific cell activities were reached particularly with E. coli BL21(DE3)/pAW-3 cells. 

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