Synthesis sites probe distributions

Figure 12. Probe distributions within synthesis sites designed to contain 10, 12, 14, 16, 18, and 20-mer probe sequences.

Figure 13. Melting curves expected for synthesis sites with the probe length distributions of Figure 12 assuming solution phase energetics, Langmuir adsorption, and 10 nM target concentration.

Figure 14. Free energy distributions for probertarget binding and probe probe associations within a given synthesis site. The details of the distributions are heuristic value only and not intended to be quantitative.

The influence of the nature of the aluminum source on the acidic properties of mesostructured materials (MCM41) has also been studied in the literature [244]. Microcalorimetry experiments using ammonia as a probe molecule have shown that Al insertion into the mesoporous silicate framework affected acid site strength and distribution in a manner controlled by the synthesis conditions (materials prepared... 

The sequence of photodeprotection and chemical coupling at match probe sites and corresponding mismatch probe sites of length L differ at only two steps out of a total of 4L synthesis steps. The probe densities and length distributions are thus expected to be quite similar. 

---