Synthesis of MAPs by fragment condensation

4-aniinobutyric add residue which allows for determination of peptide/core ratio after amino add analysis (44). 

Protocol 4. Preparation of an MAP-8 containing disulphide-linked peptide molecules 

Dissolve Fmoc-4-aminobutyric acid 1324 mg, 1 mmol) in dichloro-methane (20 ml) and add solid DCC 103 mg, 0.5 mmol) to the stirred solution. 

Filter after 40 min and evaporate the solvent under vacuum. 

Remove the Fmoc-protection with 20% v/v piperidine in DMA and perform coupling cycles by applying one of the protocols from Chapter 3 in which the following amino acid derivatives are used cycle 1 0.2 mmol Fmoc-Lys(Fmoc)-OH cycle 2. 0.3 mmol Fmoc-Lys(Fmoc)-OH cycle 3 0.4 mmol Fmoc-Lys(Fmoc)-OH cycle 4 1.0 mmol Fmoc-Glu( Bu)-OH. 

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