Synthesis of 3-D-Glucopyranoside Under Equilibrium-Controlled Condition

SYNTHESIS OF P-D-GLUCOPYRANOSIDE UNDER EQUILIBRIUM-CONTROLLED CONDITION 

Prepolymer method for entrapment of biocatalyst developed by Fukui and Tanaka is considered to be one of the best immobilization methods. Characteristic features and advantages of the prepolymer method can be summarized as follows  

Different types of gel materials, such as polysaccharides, proteins and synthetic polymers, are now used to entrap biocatalysts. Among them, photo-crosslinkable resin prepolymer ENTP-4000 as shown in Eig. 7 is more useful compared to others. Entrapment of biocatalysts should be carried out under the illumination of near ultraviolet hght within 3-5 min, by which high temperatures, shifts of pH to extremely alkahne or acidic sides are avoided. ENTP-4000, hydrophobic photo-crosslinkable resin prepolymer, is one of the most suitable prepolymers for entrapment of p-glucosidase. Molecular weight of its main chain is about 4000. 

---