Synthesis of Acyl-ACPs

This approach suffers from several disadvantages. Firstly, it requires production of apo-kCP from a strain deficient in the 4 -phosphopantetheinyl transferase sfp gene. The subsequent synthesis of acyl-ACPs is often slow and requires either production or purchase of the PPTase enzyme. Furthermore, attachment of more elaborate acyl groups requires access to synthetically challenging and expensive acyl-CoAs. 

Scheme 2.3 Workflow for the synthesis of acyl-ACPs from SNAC thioesters, and subsequent purification...

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