Surface-grafted polymer assemblies

Fig.1 Schematic illustrating the formation of surface-anchored polymer assemblies by utilizing the grafting onto and grafting from methods...

In a subsequent study, the Co coordinated assembly was grafted onto a cross-linked poly(glycidoxypropylmethacrylate) (GMA)-ethylene glycol dimethacrylate (EDMA) support surface. The template and Co ions were then leached out from the matrix. This surface grafted polymer exhibited turnover, Michaelis-Menten kinetics and mechanisms such as inhibition by tosyl phenyl alanyl chloromethyl ketone and inhibition on acetylation of hydroxyl group of 2-hydroxyethylmethacry-late (HEMA), similar to native chymotrypsin. 

Bhat R R, Chaney B N, Rowley J, Liebmann-Vinson A, Genzer J. Tailoring cell adhesion using surface-grafted polymer gradient assemblies. J Adv Mater 2005 17 2802-2807. 

For consistent and reproducible operation it is important to control chemosensor features like thickness of the MIP coatings. Generally, MIPs were prepared and immobilized on PZ resonators by using two distinct procedures, namely in situ assembling on the resonator surface [103] or physical entrapment of the preprepared MIP particles in an inert polymer matrix attached to this surface [21]. Typically, thickness of the polymer is 20 nm to 5 pm. In situ immobilization of MIPs (see below in Sects. 3.2.1-3.2.4) can be accomplished by surface grafting, sandwich casting, electropolymerization, physical entrapment or chemical coupling [102],... 

The grafting to method was used to anchor polymer chains onto the surface of silica particles and silicon wafers (Scheme 1). The synthetic procedure starts with covalent grafting of GPS to the surface. A self-assembled monolayer of GPS on silicon wafer surfaces was prepared according to the procedure suggested by Luzinov [32], For this, Si wafers were immersed in a 1% GPS toluene solution for 15 h under dry Ar atmosphere (< 1 ppm H2O). After treatment, GPS modified wafers were washed 3 times in dry toluene under dry Ar atmosphere to avoid polymerisation of non-grafted GPS and precipitation of particles. Afterwards, the silanized wafers were washed 2 times with ethanol in ultrasonic bath for 5 min followed by drying with nitrogen flux. The thickness of the GPS layer was determined by null ellipsometiy. 

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