Surface charge lactoglobulin adsorption

Surface concentrations of 1.3 mg m" for the plateau value at low bulk protein concentrations and 1.8 mg m at the higher concentrations, calculated from the surface charge density resulting from adsorption of )ff-lactoglobulin at 299 K and pH 7.0 on a platinum electrode with an anodic end potential of 0.4 V, agree very well with values in the literature... 

At pH 7.0, Roscoe et al found that surface adsorption as measured by surface charge density was at the minimum value. This may be due partly to an increase in the volume of the )8-lactoglobulin structure during this denaturation from the compact globular structures at the lower pHs, resulting in a smaller packing density and hence decreased surface concentration. At pH 8.0, their experimental results showed an increase in the... 

Protein adsorption to PS latex has also been determined by sedimentation field flow fractionation (SdFFF). The maximum surface coverages of P-casein and p-lactoglobulin on negatively charged PS latex calculated using this method were similar at around Img/m [19]. This figure, which was confirmed by amino acid analysis of the material irreversibly bound to the surface, was significantly lower... 

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