Sulfo-SMCC reaction with enzymes

The following protocol describes the activation of HRP with sulfo-SMCC. Activation of other enzymes is done similarly, with the appropriate adjustments in the mass of enzyme added to the reaction to account for differences in molecular weight. 

The gel filtration column described in step 3 should be prepared and equilibrated prior to starting the modification reaction. Enzymes preactivated with sulfo-SMCC are available from Thermo Fisher. 

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