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Sulfo-NHS acetate

It should be noted that complete blocking of all amines on proteins with sulfo-NHS acetate may cause precipitation or loss of native structure and function. The acetate modifications... [Pg.157]

Figure 1.118 Sulfo-NHS acetate may be used to block amine groups, forming permanent amide bond derivatives. Figure 1.118 Sulfo-NHS acetate may be used to block amine groups, forming permanent amide bond derivatives.
Add a 25-molar excess of sulfo-NHS acetate over the amount of amines present in the sample. If the precise amount of amines is not known, adding an equal mass of reagent to the mass of protein will provide a large excess of reactivity to completely block all amines. [Pg.158]


See other pages where Sulfo-NHS acetate is mentioned: [Pg.157]    [Pg.157]    [Pg.157]    [Pg.157]    [Pg.158]    [Pg.147]    [Pg.147]    [Pg.147]    [Pg.148]    [Pg.127]    [Pg.127]    [Pg.127]    [Pg.128]   
See also in sourсe #XX -- [ Pg.157 , Pg.158 , Pg.282 ]

See also in sourсe #XX -- [ Pg.127 ]

See also in sourсe #XX -- [ Pg.127 ]




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Sulfo-NHS

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