Sulfhydryl-reactive affinity reagents

Ellman s reagent has been used not only for the determination of sulfhydryls in proteins and other molecules, but also as a precolumn derivatization reagent for the separation of thiol compounds by HPLC (Kuwata et al., 1982), in the study of thiol-dependent enzymes (Masamune etal., 1989 Tsukamoto and Wakil, 1988 Alvear et al., 1989), and to create sulfhydryl-reactive chromatography supports for the coupling of affinity ligands (Jayabaskaran etal., 1987). Another important use of the compound... 

Figure 28.15 Two similar label transfer reagents containing a thiol-reactive methanethiolsulfonate group to label bait protein through available sulfhydryls, a tetrafluorophenyl azide group for high-efficiency photoreac-tive conjugation with interacting prey proteins, and a long biotin affinity tag.

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