Sulfhydryl-containing biomolecules

Schmidt, T. J. 1997. Helenanolide-type sesquiterpene lactones-l 11. Rates and stereochemistry in the reaction of helenalin and related helenanolides with sulfhydryl containing biomolecules. Bioorg. Med. Chem. 5, 645-653... 

The 5-iodoacetamido derivative of fluorescein (5-IAF) has been used to label numerous proteins and other biomolecules, including actin (Plank and Ware, 1987), myosin (Aguirre et al., 1986), troponin (Greene, 1986), hemoglobin (Hirsch et al., 1986), and sulfhydryl-containing proteins separated by SDS electrophoresis (Gorman, 1984). 

Mercapto-functionalized surfaces can be modified with a heterobifunctional linker in order to bind amine groups from biomolecules like, for instance, antibodies. Amino-group-containing biomolecules can be bound to that surface via maleimido-R-A-succinimidylester, which contains a sulfhydryl reactive maleimide group at one end... 

Thus, this reagent can be used to label fluorescently proteins and other biomolecules containing free sulfhydryl residues. If there are no —SH groups available, their creation can be accomplished by reduction of indigenous disulfides or through the use of various thiolation reagents (Chapter 1, Section 4.1). 

Thus, this reagent can be used to label fluorescently proteins and other biomolecules containing free sulfhydryl residues. If there are no —SH groups available. 

---