Sulfhydryl commercial preparations

Most of the bis-alkyl halides referenced in the literature are unavailable commercially, and therefore must be synthesized. Some key references to the preparation and use of these compounds for the crosslinking of sulfhydryl-containing proteins and other molecules include Goodlad (1957), Segal and Hurwitz (1976), Ewig and Kohn (1977), Wilchek and Givol (1977), Prestayko et al. (1981), Luduena et al. (1982), Hiratsuka (1988), and Aliosman et al. (1989). 

Some of the basic information on stabilizing sulfhydryl enzymes, has been responsible for their commercialization. Without the judicious use of reducing compounds throughout the processing of the papaya latex, it would not have been possible to maximize the proteolytic activity of commercial papain preparations. Examples of other studies of enzymes which have contributed to commercialization are the determination of calcium ion as a requirement for amylase stability at high temperature, the difference in properties of catalases derived from bacterial, fungal, or... 

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