Subtilisins subtilisin SSII

Fig. 10. Sequence alignment of subtilisins S41, SSII, S39, BPN, E, Carlsberg, and thermitase. Thermitase is an homologous subtilisin-like protease from the thermophilic bacterium Thermoactinomyces vulgaris. Residues conserved in four or more of the sequences are shaded. The positions of mutations discovered during the directed evolution of the various subtilisins are indicated above the alignment. E-subtilisin E, F-subtilisin S41, S-subtilisin SSII, B-subtilisin BPN. Active site residues are indicated (A).

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