Subtilisine Carlsberg esterase

A further possibility for the enzymatic removal of C-terminal blocking groups is opened up by the application of enzymes which generally display a high esterase/ protease ratio. Such a biocatalyst is the alkaline protease from Bacillus subtilis DY which shows similarities to Subtilisin Carlsberg. For this enzyme the ratio of esterase to protease activity is >105. It selectively removes methyl, ethyl and benzyl esters from a variety of Trt-, Z- and Boc-protected di- and tripeptides and a pentapeptide at pH 8 and 37 °C (Fig. 18-9) (91l... 

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