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Substrates for Sialidase Investigations

Privalova and Khorlin (1969) reported the synthesis of the p-nitrophenyl glycoside of a-N-acetylneuraminic acid. The substrate was obtained by glycosylation of p-nitrophenol with 4,7,8,9-tetra-0-acetyl-2-chloro-2-deoxy-p-N-acetylneuraminic acid methyl ester in chloroform in the presence of silver carbonate and Drierite, followed by 0-deacetylation and saponification. In enzymatic reactions, released p-nitrophenol can be determined spectrofoto-metrically. [Pg.69]

The periodate oxidation/reduction approach has also been used to differentiate between the methyl a- and p-glycosides of N-acetylneuraminic acid. Only the C7-analogue of the p-anomer could be lactonized (Yu and Ledeen 1969). The preparation of the C7-analogue of 2-deoxy-2,3-dehydro-N-acetylneuraminic acid using periodate oxidation/borohydride reduction was reported by Meindl and Tuppy (1970). [Pg.70]


See other pages where Substrates for Sialidase Investigations is mentioned: [Pg.59]    [Pg.68]   


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