Substrate mimetic-mediated synthesis

Xaa bonds catalyzed by trypsin. The improvements in the syntheses of acyl amino acid p-guanidino as well as p-amidinophenyl esters [18] promote the application of this new irreversible strategy. Furthermore, the potential of substrate mimetics mediated synthesis could be extended to other suitable proteases [19]. 

Substrate mimetic approach, substrate mimetics-mediated synthesis, an approach to protease-catalyzed peptide synthesis using substrates that mimic the specific... 

Used in the synthesis ot bioactive peptides in water-organic solvent mixtures and ionic liquids substrate-mimetic-mediated coupling ot long fragments [3-7] amino acid polymers tor biomaterials [8]... 

Variants of trypsin with increased aminolysis rate in substrate-mimetic-mediated peptide synthesis have been obtained by mutating active site residues and residues in the SI subsite. A variant called trypsiligase was described by Bordusa and coworkers [97]. It carries the mutations D189K (reduces Arg and tolerates Tyr specificity in the... 

Bordusa, R, Ullmann, D., Eisner, C., Jakubke, H.-D. (1997). Substrate mimetic mediated peptide synthesis an irreversible ligation strategy that is independent of substrate specificity. Angew. Chem. Int. Ed. Engl, 36,2473-2475. 

Gunther, R., Bordusa, F. (2000). Rrotease catalysis mediated by a substrate mimetic a novel enzymatic approach to the synthesis of carboxylic acid amides. Chemistry, 6, 463—467. 

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