Structure Determination of Slightly Branched Amyloses

Identification and Structure Determination of Slightly Branched Amyloses 

Initially, the action of maltose (3-amylase on amylose gave complete conversion into maltose,235 and amylose was considered to be a completely linear a-(l— 4) glucan. However, when highly purified crystalline sweet potato (3-amylase was used, the 

A wheat starch sample had an amylose that was the least branched, with only 27% of the molecules having a branch linkage. These branched amyloses had approximately five branch chains per molecule with an average DP of 270. The most highly-branched amylose was that obtained from sweet potato starch in which 70% of the molecules were branched, with 10 chains per molecule and an average DP of 335. 

Formation of 3-Amylase Limit Dextrins of Amylopectin and Determination of their Fine Structure 

The (3-amylase removes all glucosyl units that are part of the outer chains of amylopectin, leaving the inner part intact. The outer peripheral chains, thus, represent 

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