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Structural integrity recovery

The structural integrity of M2D-C3-0-(E0)ra-CH3 was maintained for all solid media investigated, as confirmed by qualitative analysis of the flow injection analysis (FIA) API-MS spectra (example shown in Fig. 2.8.10a). Intercalation of the surfactant solution between the clay layers was considered as the likely cause of the reduced recovery on montmorillonite (74%), according to experimental observations and... [Pg.660]

Cytotoxicity assays can be performed in various ways. In some cases, alterations of metabolic activities induced by drugs are measured. In other cases, structural integrity, which may or may not be directly related to cell death, is evaluated. Alternatively, cell survival assays measuring the result of each metabolic perturbation can be performed and used to evaluate cell recovery or death. [Pg.35]

Triarhou LC (1996) The cerebellar model of neural grafting Structural integration and functional recovery. Brain Res Bull 59 127-138. [Pg.299]

Table XVIII provides an estimate of the structural integrity of the SNF containers, at the present time (1996) and in the year 2015, and implications for recovery purposes. This represents a theoretical assessment only, and should therefore not suggest remedial action without confirmation of the actual condition of the SNF containers through site survey. Table XVIII provides an estimate of the structural integrity of the SNF containers, at the present time (1996) and in the year 2015, and implications for recovery purposes. This represents a theoretical assessment only, and should therefore not suggest remedial action without confirmation of the actual condition of the SNF containers through site survey.
Recombinant DNA techniques are widely used in the production of therapeutic proteins, and the problems associated with solubility, structural integrity and biological activity, and contamination with host proteins have to be reduced to a minimum. Consequently, the development of fast, reliable analytical assays is essential for monitoring the progress of a purification process and assessing its effectiveness (yield, biological activity, recovery) [4]. [Pg.151]

WSRC, WSRC-TR-90-321, "Structural Integrity Assessment of the Moderator Recovery System K-, P-, and L-Reactors - Savannah River," Sept., 1990. [Pg.318]

Following successful recovery of peptide/protein molecule from the microspheres, a simple spectrophotometric method does not always allow discrimination between the monomeric protein form and its aggregates. However, HPLC might separate these species and thus provides more accurate qualitative data [96], But HPLC cannot quantify exclusively the amount of active protein antigen, as is the case with ELISA techniques [97], Nowadays, Fourier transform infrared (FTIR) spectroscopy has become a popular, noninvasive method, as it is able to characterize the secondary structure of entrapped proteins [26, 95, 98-101], Only recently, the integrity of their primary structure was evaluated, thanks to a new matrix-assisted laser... [Pg.406]

Both (IV) and (V) are stable in nonpolar solvents but not in acidic or basic solutions 127, 128). Similarly, the integrity of YCN is retained, but only in the structurally intact protein, in the absence of potential enzyme ligands (formic, acetic, or hydrofluoric acids). Such ligands promote a slow hydrolysis of YCN (Table VI) with a concomitant recovery of full enzymic activity 90). Furthermore, acid denaturation of the cyanylated enzyme at pH 2 is followed by complete hydrolysis of YCN (half-time 4 hr at pH 2, 25°) 90), and the formation of carbon dioxide, possibly via Eqs. (8) and (9) ... [Pg.383]


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