Streptomyces glaucescens Tetracenomycins

B Shen, RG Summers, H Gramajo, MJ Bibb, CR Hutchinson. Purification and characterization of the acyl carrier protein of the Streptomyces glaucescens tetracenomycin C polyketide synthase. JBacteriol 174 3818-3821, 1992. 

Figure 6 Cell-free biosynthesis of tetracenomycin F2. A strain of Streptomyces glaucescens in which the tetracenomycin-minimal PKS genes and an ARO/CYC activity had been overexpressed produced tetracenomycin F2, a precursor to the natural product tetracenomycin A (the remainder of the pathway is indicated by dashed arrows). The same result has been achieved using purified proteins in vitro.

HC Gramajo, J White, CR Hutchinson, MJ Bibb. Overproduction and localization of components of the polyketide synthase of Streptomyces glaucescens involved in the production ofthe antibiotic tetracenomycin C. JBacteriol 173 6475-6483,1991. 

SUMMERS, R.G., WENDT-PIENKOWSKI, E., MOTAMEDI, H HUTCHINSON, C.R., Nucleotide sequence of the tcmll-tcmlV region of the tetracenomycin C biosynthetic gene cluster of Streptomyces glaucescens and evidence that the tcmN gene encodes a multifunctional cyclase-dehydratase-O-methyl transferase, J. Bacteriol., 1992,174, 1810-1820. 

Motamedi, H. and C.R. Hutchinson (1987). Cloning and heterologous expression of a gene cluster for the biosynthesis of tetracenomycin C, the anthracycline antitumor antibiotic of Streptomyces glaucescens. Proc Natl Acad Sci U S A 84 4445-4449. 

Summers, R.G., E. Wendt-Pienkowsky, H. Motamedi, C.R. Hutchinson, CR. 1993. The tcmVI region of the tetracenomycin C biosynthetic gene duster of Streptomyces glaucescens encodes the tetracenomycin FI monooxygenase, tetracenomycin F2 cyclase, and most likely, a second cyclase. J. Bacteriol. 175 7571-7580. 

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